Unknown

Dataset Information

0

Conformational change of syntaxin linker region induced by Munc13s initiates SNARE complex formation in synaptic exocytosis.


ABSTRACT: The soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein syntaxin-1 adopts a closed conformation when bound to Munc18-1, preventing binding to synaptobrevin-2 and SNAP-25 to form the ternary SNARE complex. Although it is known that the MUN domain of Munc13-1 catalyzes the transition from the Munc18-1/syntaxin-1 complex to the SNARE complex, the molecular mechanism is unclear. Here, we identified two conserved residues (R151, I155) in the syntaxin-1 linker region as key sites for the MUN domain interaction. This interaction is essential for SNARE complex formation in vitro and synaptic vesicle priming in neuronal cultures. Moreover, this interaction is important for a tripartite Munc18-1/syntaxin-1/MUN complex, in which syntaxin-1 still adopts a closed conformation tightly bound to Munc18-1, whereas the syntaxin-1 linker region changes its conformation, similar to that of the LE mutant of syntaxin-1 when bound to Munc18-1. We suggest that the conformational change of the syntaxin-1 linker region induced by Munc13-1 initiates ternary SNARE complex formation in the neuronal system.

SUBMITTER: Wang S 

PROVIDER: S-EPMC5350566 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

Conformational change of syntaxin linker region induced by Munc13s initiates SNARE complex formation in synaptic exocytosis.

Wang Shen S   Choi Ucheor B UB   Gong Jihong J   Yang Xiaoyu X   Li Yun Y   Wang Austin L AL   Yang Xiaofei X   Brunger Axel T AT   Ma Cong C  

The EMBO journal 20170130 6


The soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein syntaxin-1 adopts a closed conformation when bound to Munc18-1, preventing binding to synaptobrevin-2 and SNAP-25 to form the ternary SNARE complex. Although it is known that the MUN domain of Munc13-1 catalyzes the transition from the Munc18-1/syntaxin-1 complex to the SNARE complex, the molecular mechanism is unclear. Here, we identified two conserved residues (R151, I155) in the syntaxin-1 linker region  ...[more]

Similar Datasets

| S-EPMC9166750 | biostudies-literature
| S-EPMC5860373 | biostudies-literature
| S-EPMC10911398 | biostudies-literature
| S-EPMC4955600 | biostudies-literature
| S-EPMC2697323 | biostudies-literature
| S-EPMC3235364 | biostudies-literature
| S-EPMC2063557 | biostudies-literature
| S-EPMC1446092 | biostudies-literature
| S-EPMC7363831 | biostudies-literature
| S-EPMC2765074 | biostudies-literature