Identification of Filamin-A and -B as potential biomarkers for prostate cancer.
Ontology highlight
ABSTRACT: Aim
A novel strategy for prostate cancer (PrCa) biomarker discovery is described.Materials & methods
In vitro perturbation biology, proteomics and Bayesian causal analysis identified biomarkers that were validated in in vitro models and clinical specimens.Results
Filamin-B (FLNB) and Keratin-19 were identified as biomarkers. Filamin-A (FLNA) was found to be causally linked to FLNB. Characterization of the biomarkers in a panel of cells revealed differential mRNA expression and regulation. Moreover, FLNA and FLNB were detected in the conditioned media of cells. Last, in patients without PrCa, FLNA and FLNB blood levels were positively correlated, while in patients with adenocarcinoma the relationship is dysregulated.Conclusion
These data support the strategy and the potential use of the biomarkers for PrCa.
SUBMITTER: Narain NR
PROVIDER: S-EPMC5351499 | biostudies-literature | 2017 Mar
REPOSITORIES: biostudies-literature
Publications
Identification of Filamin-A and -B as potential biomarkers for prostate cancer.
Narain Niven R NR Diers Anne R AR Lee Arleide A Lao Socheata S Chan Joyce Y JY Schofield Sally S Andreazi Joe J Ouro-Djobo Rakibou R Jimenez Joaquin J JJ Friss Tracey T Tanna Nikunj N Dalvi Aditee A Wang Sihe S Bunch Dustin D Sun Yezhou Y Wu Wenfang W Thapa Khampaseuth K Gesta Stephane S Rodrigues Leonardo O LO Akmaev Viatcheslav R VR Vishnudas Vivek K VK Sarangarajan Rangaprasad R
Future science OA 20161222 1
<h4>Aim</h4>A novel strategy for prostate cancer (PrCa) biomarker discovery is described.<h4>Materials & methods</h4><i>In vitro</i> perturbation biology, proteomics and Bayesian causal analysis identified biomarkers that were validated in <i>in vitro</i> models and clinical specimens.<h4>Results</h4>Filamin-B (FLNB) and Keratin-19 were identified as biomarkers. Filamin-A (FLNA) was found to be causally linked to FLNB. Characterization of the biomarkers in a panel of cells revealed differential ...[more]
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