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Flow cytometry-assisted cloning of specific sequence motifs from complex 16S rRNA gene libraries.

ABSTRACT: A flow cytometry method was developed for rapid screening and recovery of cloned DNA containing common sequence motifs. This approach, termed fluorescence-activated cell sorting-assisted cloning, was used to recover sequences affiliated with a unique lineage within the Bacteroidetes not abundant in a clone library of environmental 16S rRNA genes.

SUBMITTER: Nielsen JL

PROVIDER: S-EPMC535210 | biostudies-literature | 2004 Dec

REPOSITORIES: biostudies-literature

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Flow cytometry-assisted cloning of specific sequence motifs from complex 16S rRNA gene libraries.

Nielsen Jeppe L JL Schramm Andreas A Bernhard Anne E AE van den Engh Gerrit J GJ Stahl David A DA

Applied and environmental microbiology 20041201 12

A flow cytometry method was developed for rapid screening and recovery of cloned DNA containing common sequence motifs. This approach, termed fluorescence-activated cell sorting-assisted cloning, was used to recover sequences affiliated with a unique lineage within the Bacteroidetes not abundant in a clone library of environmental 16S rRNA genes. ...[more]

PMID: 15574959

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