Ontology highlight
ABSTRACT:
SUBMITTER: Suzuki M
PROVIDER: S-EPMC535262 | biostudies-literature | 2004 Dec
REPOSITORIES: biostudies-literature
Suzuki Masahiro M Matsumoto Masakado M Hata Mami M Takahashi Masao M Sakae Kenji K
Journal of clinical microbiology 20041201 12
We developed a rapid PCR method utilizing the diversity of the insertion site IS1203 for genotyping Shiga toxin-producing Escherichia coli (STEC) O157 (IS1203 PCR typing). DNA fragments digested by PvuII, which cut IS1203 at one site, were ligated with themselves and detected by PCR with outward-facing primer pairs for IS1203. To minimize nonspecific bands, nested PCR was also performed. Two fingerprinting patterns produced from the upstream or downstream regions of IS1203 were obtained within 1 ...[more]