Detection of the van alphabet and identification of enterococci and staphylococci at the species level by multiplex PCR.
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ABSTRACT:
SUBMITTER: Depardieu F
PROVIDER: S-EPMC535300 | biostudies-literature | 2004 Dec
REPOSITORIES: biostudies-literature
Publications
Detection of the van alphabet and identification of enterococci and staphylococci at the species level by multiplex PCR.
Depardieu Florence F Perichon Bruno B Courvalin Patrice P
Journal of clinical microbiology 20041201 12
A multiplex PCR assay was developed for detection of the six types of glycopeptide resistance characterized in enterococci and for identification of Enterococcus faecium, Enterococcus faecalis, Staphylococcus aureus, and Staphylococcus epidermidis at the species level. Primers targeting the genes vanA, vanB, vanC, vanD, vanE, vanG, and ddl of E. faecium and E. faecalis and nuc of S. aureus and a chromosomal portion specific to S. epidermidis were designed to allow amplification of fragments with ...[more]
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