CD8⁺ T cells specific for the islet autoantigen IGRP are restricted in their T cell receptor chain usage.

ABSTRACT: CD8⁺ T cells directed against beta cell autoantigens are considered relevant for the pathogenesis of type 1 diabetes. Using single cell T cell receptor sequencing of CD8⁺ T cells specific for the IGRP_265-273 epitope, we examined whether there was expansion of clonotypes and sharing of T cell receptor chains in autoreactive CD8⁺ T cell repertoires. HLA-A*0201 positive type 1 diabetes patients (n = 19) and controls (n = 18) were analysed. TCR α- and β-chain sequences of 418 patient-derived IGRP_265-273-multimer⁺ CD8⁺ T cells representing 48 clonotypes were obtained. Expanded populations of IGRP_265-273-specific CD8⁺ T cells with dominant clonotypes that had TCR α-chains shared across patients wer

SUBMITTER: Fuchs YF 

PROVIDER: S-EPMC5353542 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature