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High-resolution phenotypic profiling of natural products-induced effects on the single-cell level.

ABSTRACT: Natural products (NPs) are highly evolved molecules making them a valuable resource for new therapeutics. Here we demonstrate the usefulness of broad-spectrum phenotypic profiling of NP-induced perturbations on single cells with imaging-based High-Content Screening to inform on physiology, mechanisms-of-actions, and multi-level toxicity. Our technology platform aims at broad applicability using a comprehensive marker panel with standardized settings streamlined towards an easy implementation in laboratories dedicated to natural products research.

SUBMITTER: Kremb S

PROVIDER: S-EPMC5353608 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

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High-resolution phenotypic profiling of natural products-induced effects on the single-cell level.

Kremb Stephan S Voolstra Christian R CR

Scientific reports 20170315

Natural products (NPs) are highly evolved molecules making them a valuable resource for new therapeutics. Here we demonstrate the usefulness of broad-spectrum phenotypic profiling of NP-induced perturbations on single cells with imaging-based High-Content Screening to inform on physiology, mechanisms-of-actions, and multi-level toxicity. Our technology platform aims at broad applicability using a comprehensive marker panel with standardized settings streamlined towards an easy implementation in ...[more]

PMID: 28295057

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