Fast detection of tobacco mosaic virus infected tobacco using laser-induced breakdown spectroscopy.

ABSTRACT: Tobacco mosaic virus (TMV) is one of the most devastating viruses to crops, which can cause severe production loss and affect the quality of products. In this study, we have proposed a novel approach to discriminate TMV-infected tobacco based on laser-induced breakdown spectroscopy (LIBS). Two different kinds of tobacco samples (fresh leaves and dried leaf pellets) were collected for spectral acquisition, and partial least squared discrimination analysis (PLS-DA) was used to establish classification models based on full spectrum and observed emission lines. The influences of moisture content on spectral profile, signal stability and plasma parameters (temperature and electron density) were also analysed. The results revealed that moisture content in fresh tobacco leaves would worsen the st

SUBMITTER: Peng J 

PROVIDER: S-EPMC5353609 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature