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Genome-wide analysis of complex wheat gliadins, the dominant carriers of celiac disease epitopes.


ABSTRACT: Gliadins, specified by six compound chromosomal loci (Gli-A1/B1/D1 and Gli-A2/B2/D2) in hexaploid bread wheat, are the dominant carriers of celiac disease (CD) epitopes. Because of their complexity, genome-wide characterization of gliadins is a strong challenge. Here, we approached this challenge by combining transcriptomic, proteomic and bioinformatic investigations. Through third-generation RNA sequencing, full-length transcripts were identified for 52 gliadin genes in the bread wheat cultivar Xiaoyan 81. Of them, 42 were active and predicted to encode 25 ?-, 11 ?-, one ?- and five ?-gliadins. Comparative proteomic analysis between Xiaoyan 81 and six newly-developed mutants each lacking one Gli locus indicated the accumulation of 38 gliadins in the mature grains. A novel group of ?-gliadins (the CSTT group) was recognized to contain very few or no CD epitopes. The ?-gliadins identified here or previously did not carry CD epitopes. Finally, the mutant lacking Gli-D2 showed significant reductions in the most celiac-toxic ?-gliadins and derivative CD epitopes. The insights and resources generated here should aid further studies on gliadin functions in CD and the breeding of healthier wheat.

SUBMITTER: Wang DW 

PROVIDER: S-EPMC5353739 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

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Genome-wide analysis of complex wheat gliadins, the dominant carriers of celiac disease epitopes.

Wang Da-Wei DW   Li Da D   Wang Junjun J   Zhao Yue Y   Wang Zhaojun Z   Yue Guidong G   Liu Xin X   Qin Huanju H   Zhang Kunpu K   Dong Lingli L   Wang Daowen D  

Scientific reports 20170316


Gliadins, specified by six compound chromosomal loci (Gli-A1/B1/D1 and Gli-A2/B2/D2) in hexaploid bread wheat, are the dominant carriers of celiac disease (CD) epitopes. Because of their complexity, genome-wide characterization of gliadins is a strong challenge. Here, we approached this challenge by combining transcriptomic, proteomic and bioinformatic investigations. Through third-generation RNA sequencing, full-length transcripts were identified for 52 gliadin genes in the bread wheat cultivar  ...[more]

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