Dataset Information

Influence of a pre-stimulation with chronic low-dose UVB on stress response mechanisms in human skin fibroblasts.

ABSTRACT: Exposure to solar ultraviolet type B (UVB), through the induction of cyclobutane pyrimidine dimer (CPD), is the major risk factor for cutaneous cancer. Cells respond to UV-induced CPD by triggering the DNA damage response (DDR) responsible for signaling DNA repair, programmed cell death and cell cycle arrest. Underlying mechanisms implicated in the DDR have been extensively studied using single acute UVB irradiation. However, little is known concerning the consequences of chronic low-dose of UVB (CLUV) on the DDR. Thus, we have investigated the effect of a CLUV pre-stimulation on the different stress response pathways. We found that CLUV pre-stimulation enhances CPD repair capacity and leads to a cell cycle delay but leave residual unrepaired CPD. We further analyzed the consequence of the CLUV regimen on general gene and protein expression. We found that CLUV treatment influences biological processes related to the response to stress at the transcriptomic and proteomic levels. This overview study represents the first demonstration that human cells respond to chronic UV irradiation by modulating their genotoxic stress response mechanisms.

SUBMITTER: Drigeard Desgarnier MC

PROVIDER: S-EPMC5354420 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Influence of a pre-stimulation with chronic low-dose UVB on stress response mechanisms in human skin fibroblasts.

Drigeard Desgarnier Marie-Catherine MC Fournier Frédéric F Droit Arnaud A Rochette Patrick J PJ

PloS one 20170316 3

Exposure to solar ultraviolet type B (UVB), through the induction of cyclobutane pyrimidine dimer (CPD), is the major risk factor for cutaneous cancer. Cells respond to UV-induced CPD by triggering the DNA damage response (DDR) responsible for signaling DNA repair, programmed cell death and cell cycle arrest. Underlying mechanisms implicated in the DDR have been extensively studied using single acute UVB irradiation. However, little is known concerning the consequences of chronic low-dose of UVB ...[more]

PMID: 28301513

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Dataset Information

Influence of a pre-stimulation with chronic low-dose UVB on stress response mechanisms in human skin fibroblasts.

Publications

Influence of a pre-stimulation with chronic low-dose UVB on stress response mechanisms in human skin fibroblasts.

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