Unknown

Dataset Information

0

Promotion Effects of miR-375 on the Osteogenic Differentiation of Human Adipose-Derived Mesenchymal Stem Cells.


ABSTRACT: MicroRNA plays an important role in bone tissue engineering; however, its role and function in osteogenic differentiation warrant further investigation. In this study, we demonstrated that miR-375 was upregulated during the osteogenic differentiation of human adipose-derived mesenchymal stem cells (hASCs). Overexpression of miR-375 significantly enhanced hASCs osteogenesis both in vitro and in vivo, while knockdown of miR-375 inhibited the osteogenic differentiation of hASCs. Mechanistically, microarray analysis revealed DEPTOR as a target of miR-375 in hASCs. Knockdown of DEPTOR accelerated the osteogenic differentiation of hASCs by inhibiting AKT signaling, which mimics miR-375 overexpression. Furthermore, we confirmed that miR-375 regulated osteogenesis by targeting YAP1, and that YAP1 reversely bound to miR-375 promoter to inhibit miR-375 expression. Taken together, our results suggested that miR-375 promoted the osteogenic differentiation of hASCs via the YAP1/DEPTOR/AKT regulatory network, indicating that miR-375-targeted therapy might be a valuable approach to promote bone regeneration.

SUBMITTER: Chen S 

PROVIDER: S-EPMC5355733 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

Promotion Effects of miR-375 on the Osteogenic Differentiation of Human Adipose-Derived Mesenchymal Stem Cells.

Chen Si S   Zheng Yunfei Y   Zhang Shan S   Jia Lingfei L   Zhou Yongsheng Y  

Stem cell reports 20170302 3


MicroRNA plays an important role in bone tissue engineering; however, its role and function in osteogenic differentiation warrant further investigation. In this study, we demonstrated that miR-375 was upregulated during the osteogenic differentiation of human adipose-derived mesenchymal stem cells (hASCs). Overexpression of miR-375 significantly enhanced hASCs osteogenesis both in vitro and in vivo, while knockdown of miR-375 inhibited the osteogenic differentiation of hASCs. Mechanistically, mi  ...[more]

Similar Datasets

| S-EPMC6797519 | biostudies-literature
| S-EPMC8258422 | biostudies-literature
| S-EPMC5636128 | biostudies-literature
| S-EPMC9495020 | biostudies-literature
| S-EPMC7408391 | biostudies-literature
| S-EPMC7687013 | biostudies-literature
| S-EPMC7448845 | biostudies-literature
| S-EPMC10481475 | biostudies-literature
| S-EPMC6237555 | biostudies-literature
| S-EPMC6496323 | biostudies-literature