Ontology highlight
ABSTRACT:
SUBMITTER: Mahon BP
PROVIDER: S-EPMC5358508 | biostudies-literature | 2015 Nov
REPOSITORIES: biostudies-literature
Mahon Brian P BP Díaz-Torres Natalia A NA Pinard Melissa A MA Tu Chingkuang C Silverman David N DN Scott Kathleen M KM McKenna Robert R
Bioorganic & medicinal chemistry letters 20150506 21
Thiomicrospira crunogena XCL-2 expresses an α-carbonic anhydrase (TcruCA). Sequence alignments reveal that TcruCA displays a high sequence identity (>30%) relative to other α-CAs. This includes three conserved histidines that coordinate the active site zinc, a histidine proton shuttling residue, and opposing hydrophilic and hydrophobic sides that line the active site. The catalytic efficiency of TcruCA is considered moderate relative to other α-CAs (k(cat)/K(M)=1.1×10(7) M(-1) s(-1)), being a fa ...[more]