Dataset Information

Genome-Guided Insights into the Plant Growth Promotion Capabilities of the Physiologically Versatile Bacillus aryabhattai Strain AB211.

ABSTRACT: Bacillus aryabhattai AB211 is a plant growth promoting, Gram-positive firmicute, isolated from the rhizosphere of tea (Camellia sinensis), one of the oldest perennial crops and a major non-alcoholic beverage widely consumed all over the world. The whole genome of B. aryabhattai AB211 was sequenced, annotated and evaluated with special focus on genomic elements related to plant microbe interaction. It's genome sequence reveals the presence of a 5,403,026 bp chromosome. A total of 5226 putative protein-coding sequences, 16 rRNA, 120 tRNA, 8 ncRNAs, 58 non-protein coding genes, and 11 prophage regions were identified. Genome sequence comparisons between strain AB211 and other related environmental strains of B. aryabhattai, identified about 3558 genes conserved among all B. aryabhattai genomes analyzed. Most of the common genes involved in plant growth promotion activities were found to be present within core genes of all the genomes used for comparison, illustrating possible common plant growth promoting traits shared among all the strains of B. aryabhattai. Besides the core genes, some genes were exclusively identified in the genome of strain AB211. Functional annotation of the genes predicted in the strain AB211 revealed the presence of genes responsible for mineral phosphate solubilization, siderophores, acetoin, butanediol, exopolysaccharides, flagella biosynthesis, surface attachment/biofilm formation, and indole acetic acid production, most of which were experimentally verified in the present study. Genome analysis and experimental evidence suggested that AB211 has robust central carbohydrate metabolism implying that this bacterium can efficiently utilize the root exudates and other organic materials as an energy source. Genes for the production of peroxidases, catalases, and superoxide dismutases, that confer resistance to oxidative stresses in plants were identified in AB211 genome. Besides these, genes for heat shock tolerance, cold shock tolerance, glycine-betaine production, and antibiotic/heavy metal resistance that enable bacteria to survive biotic/abiotic stress were also identified. Based on the genome sequence information and experimental evidence as presented in this study, strain AB211 appears to be metabolically diverse and exhibits tremendous potential as a plant growth promoting bacterium.

SUBMITTER: Bhattacharyya C

PROVIDER: S-EPMC5359284 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Genome-Guided Insights into the Plant Growth Promotion Capabilities of the Physiologically Versatile Bacillus aryabhattai Strain AB211.

Bhattacharyya Chandrima C Bakshi Utpal U Mallick Ivy I Mukherji Shayantan S Bera Biswajit B Ghosh Abhrajyoti A

Frontiers in microbiology 20170321

Bacillus aryabhattai AB211 is a plant growth promoting, Gram-positive firmicute, isolated from the rhizosphere of tea (Camellia sinensis), one of the oldest perennial crops and a major non-alcoholic beverage widely consumed all over the world. The whole genome of B. aryabhattai AB211 was sequenced, annotated and evaluated with special focus on genomic elements related to plant microbe interaction. It's genome sequence reveals the presence of a 5,403,026 bp chromosome. A tota ...[more]

PMID: 28377746

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Project description:Plant growth-promoting rhizobacteria (PGPR) can produce hormone-like substances, promote plant nutrient uptake, enhance plant resistance, inhibit the growth of pathogenic bacteria, and induce plant resistance to biotic and abiotic stresses. Bacillus is one of the most studied genera that promote plant root development. Since its discovery in 2009, B. aryabhattai has shown promising properties such as promoting plant growth and improving crop yield. However, the mechanisms of B. aryabhattai promoting plant growth remain to be investigated. In this study, the chromosome of B. aryabhattai strain LAD and five plasmids within the cell were sequenced and annotated. The genome, with a length of 5,194,589 bp and 38.12% GC content, contains 5,288 putative protein-coding genes, 39 rRNA, and 112 tRNA. The length of the five plasmids ranged from 116,519 to 212,484 bp, and a total of 810 putative protein-coding genes, 4 rRNA, and 32 tRNA were predicted in the plasmids. Functional annotation of the predicted genes revealed numerous genes associated with indole-3 acetic acid (IAA) and exopolysaccharides (EPSs) biosynthesis, membrane transport, nitrogen cycle metabolism, signal transduction, cell mobility, stress response, and antibiotic resistance on the genome which benefits the plants. Genes of carbohydrate-active enzymes were detected in both the genome and plasmids suggesting that LAD has the capacity of synthesizing saccharides and utilizing organic materials like root exudates. LAD can utilize different carbon sources of varied carbon chain length, i.e., methanol, acetate, glycerol, glucose, sucrose, and starch for growth and temperature adaptation suggesting a high versatility of LAD for thriving in fluctuating environments. LAD produced the most EPSs with sucrose as sole carbon source, and high concentration of IAA was produced when the maize plant was cultivated with LAD, which may enhance plant growth. LAD significantly stimulated the development of the maize root. The genome-based information and experimental evidence demonstrated that LAD with diverse metabolic capabilities and positive interactions with plants has tremendous potential for adaptation to the dynamic soil environments and promoting plant growth.

Project description:Plant growth-promoting bacteria (PGPB) appear to be a sensible competitor to conventional fertilization, including mineral fertilizers and chemical plant protection products. Undoubtedly, one of the most interesting bacteria exhibiting plant-stimulating traits is, more widely known as a pathogen, Bacillus cereus. To date, several environmentally safe strains of B. cereus have been isolated and described, including B. cereus WSE01, MEN8, YL6, SA1, ALT1, ERBP, GGBSTD1, AK1, AR156, C1L, and T4S. These strains have been studied under growth chamber, greenhouse, and field conditions and have shown many significant traits, including indole-3-acetic acid (IAA) and aminocyclopropane-1-carboxylic acid (ACC) deaminase production or phosphate solubilization, which allows direct plant growth promotion. It includes an increase in biometrics traits, chemical element content (e.g., N, P, and K), and biologically active substances content or activity, e.g., antioxidant enzymes and total soluble sugar. Hence, B. cereus has supported the growth of plant species such as soybean, maize, rice, and wheat. Importantly, some B. cereus strains can also promote plant growth under abiotic stresses, including drought, salinity, and heavy metal pollution. In addition, B. cereus strains produced extracellular enzymes and antibiotic lipopeptides or triggered induced systemic resistance, which allows indirect stimulation of plant growth. As far as biocontrol is concerned, these PGPB can suppress the development of agriculturally important phytopathogens, including bacterial phytopathogens (e.g., Pseudomonas syringae, Pectobacterium carotovorum, and Ralstonia solanacearum), fungal phytopathogens (e.g., Fusarium oxysporum, Botrytis cinerea, and Rhizoctonia solani), and other phytopathogenic organisms (e.g., Meloidogyne incognita (Nematoda) and Plasmodiophora brassicae (Protozoa)). In conclusion, it should be noted that there are still few studies on the effectiveness of B. cereus under field conditions, particularly, there is a lack of comprehensive analyses comparing the PGP effects of B. cereus and mineral fertilizers, which should be reduced in favor of decreasing the use of mineral fertilizers. It is also worth mentioning that there are still very few studies on the impact of B. cereus on the indigenous microbiota and its persistence after application to soil. Further studies would help to understand the interactions between B. cereus and indigenous microbiota, subsequently contributing to increasing its effectiveness in promoting plant growth.

Dataset Information

Genome-Guided Insights into the Plant Growth Promotion Capabilities of the Physiologically Versatile Bacillus aryabhattai Strain AB211.

Publications

Genome-Guided Insights into the Plant Growth Promotion Capabilities of the Physiologically Versatile <i>Bacillus aryabhattai</i> Strain AB211.

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