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Protection of Human Umbilical Vein Endothelial Cells against Oxidative Stress by MicroRNA-210.

ABSTRACT: Oxidative stress induces endothelial cell apoptosis and promotes atherosclerosis development. MicroRNA-210 (miR-210) is linked with apoptosis in different cell types. This study aimed to investigate the role of miR-210 in human umbilical vein endothelial cells (HUVECs) under oxidative stress and to determine the underlying mechanism. HUVECs were treated with different concentrations of hydrogen peroxide (H₂O₂), and cell viability was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and ATP assay. To evaluate the role of miR-210 in H₂O₂-mediated apoptosis, gain-and-loss-of-function approaches were used, and the effects on apoptosis and reactive oxygen species (ROS) level were assayed using flow cytometry. Moreover, miR-210 expression was detected by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), and expression of the following apoptosis-related genes was assessed by qRT-PCR and Western blot at the RNA and protein level, respectively: caspase-8-associated protein 2 (CASP8AP2), caspase-8, and caspase-3. The results showed that H₂O₂ induced apoptosis in HUVECs in a dose-dependent manner and increased miR-210 expression. Overexpression of miR-210 inhibited apoptosis and reduced ROS level in HUVECs treated with H₂O₂. Furthermore, miR-210 downregulated CASP8AP2 and related downstream caspases at protein level. Thus, under oxidative stress, miR-210 has a prosurvival and antiapoptotic effect on HUVECs by reducing ROS generation and downregulating the CASP8AP2 pathway.

SUBMITTER: Li T

PROVIDER: S-EPMC5359453 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Protection of Human Umbilical Vein Endothelial Cells against Oxidative Stress by MicroRNA-210.

Li Tianyi T Song Xianjing X Zhang Jichang J Zhao Lei L Shi Yongfeng Y Li Zhibo Z Liu Jia J Liu Ning N Yan Youyou Y Xiao Yanlong Y Tian Xin X Sun Wei W Guan Yinuo Y Liu Bin B

Oxidative medicine and cellular longevity 20170307

Oxidative stress induces endothelial cell apoptosis and promotes atherosclerosis development. MicroRNA-210 (miR-210) is linked with apoptosis in different cell types. This study aimed to investigate the role of miR-210 in human umbilical vein endothelial cells (HUVECs) under oxidative stress and to determine the underlying mechanism. HUVECs were treated with different concentrations of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), and cell viability was evaluated using the 3-(4,5-dimethylthiaz ...[more]

PMID: 28367268

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Protection of Human Umbilical Vein Endothelial Cells against Oxidative Stress by MicroRNA-210.

Publications

Protection of Human Umbilical Vein Endothelial Cells against Oxidative Stress by MicroRNA-210.

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