Multistep regulation of TFEB by MTORC1.
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ABSTRACT:
SUBMITTER: Vega-Rubin-de-Celis S
PROVIDER: S-EPMC5361595 | biostudies-literature | 2017 Mar
REPOSITORIES: biostudies-literature
Publications
Multistep regulation of TFEB by MTORC1.
Vega-Rubin-de-Celis Silvia S Peña-Llopis Samuel S Konda Meghan M Brugarolas James J
Autophagy 20170105 3
The master regulator of lysosome biogenesis, TFEB, is regulated by MTORC1 through phosphorylation at S211, and a S211A mutation increases nuclear localization. However, TFEB<sup>S211A</sup> localizes diffusely in both cytoplasm and nucleus and, as we show, retains regulation by MTORC1. Here, we report that endogenous TFEB is phosphorylated at S122 in an MTORC1-dependent manner, that S122 is phosphorylated in vitro by recombinant MTOR, and that S122 is important for TFEB regulation by MTORC1. Spe ...[more]
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2011-08-02 | E-GEOD-28021 | biostudies-arrayexpress