Ontology highlight
ABSTRACT:
SUBMITTER: Vega-Rubin-de-Celis S
PROVIDER: S-EPMC5361595 | biostudies-literature | 2017 Mar
REPOSITORIES: biostudies-literature
Autophagy 20170105 3
The master regulator of lysosome biogenesis, TFEB, is regulated by MTORC1 through phosphorylation at S211, and a S211A mutation increases nuclear localization. However, TFEB<sup>S211A</sup> localizes diffusely in both cytoplasm and nucleus and, as we show, retains regulation by MTORC1. Here, we report that endogenous TFEB is phosphorylated at S122 in an MTORC1-dependent manner, that S122 is phosphorylated in vitro by recombinant MTOR, and that S122 is important for TFEB regulation by MTORC1. Spe ...[more]