Ontology highlight
ABSTRACT:
SUBMITTER: Usarek E
PROVIDER: S-EPMC5362213 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
Usarek Ewa E Barańczyk-Kuźma Anna A Kaźmierczak Beata B Gajewska Beata B Kuźma-Kozakiewicz Magdalena M
PloS one 20170322 3
Quantitative polymerase chain reaction (qPCR) is the most specific and reliable method for determination of mRNA gene expression. Crucial point for its accurate normalization is the choice of appropriate internal control genes (ICGs). In the present work we determined and compare the expression of eight commonly used ICGs in lymphocytes from 26 patients with amyotrophic lateral sclerosis (ALS) and 30 control subjects. Peripheral blood mononuclear cells (PBMCs) before and after immortalization by ...[more]