Macrophage Polarization Modulates FcγR- and CD13-Mediated Phagocytosis and Reactive Oxygen Species Production, Independently of Receptor Membrane Expression.

ABSTRACT: In response to microenvironmental cues, macrophages undergo a profound phenotypic transformation acquiring distinct activation phenotypes ranging from pro-inflammatory (M1) to anti-inflammatory (M2). To study how activation phenotype influences phagocytosis and production of reactive oxygen species (ROS) mediated by receptors for IgG antibodies (Fcγ receptors) and by CD13, human monocyte-derived macrophages were polarized to distinct phenotypes using IFN-γ (Mϕ-IFN-γ), IL-4 (Mϕ-IL-4), or IL-10 (Mϕ-IL-10). Phenotypically, Mϕ-IFN-γ were characterized as CD14⁺CD80⁺CD86⁺ cells, Mϕ-IL-4 as CD209^highCD206⁺CD11b⁺CD14^low, and Mϕ-IL-10 as CD16⁺CD163⁺ cells. Compared to non-polarized macrophages, FcγRI

SUBMITTER: Mendoza-Coronel E 

PROVIDER: S-EPMC5366847 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature