Unknown

Dataset Information

0

A novel method of sampling gingival crevicular fluid from a mouse model of periodontitis.


ABSTRACT: Using a mouse model of silk ligature-induced periodontal disease (PD), we report a novel method of sampling mouse gingival crevicular fluid (GCF) to evaluate the time-dependent secretion patterns of bone resorption-related cytokines. GCF is a serum transudate containing host-derived biomarkers which can represent cellular response in the periodontium. As such, human clinical evaluations of PD status rely on sampling this critical secretion. At the same time, a method of sampling GCF from mice is absent, hindering the translational value of mouse models of PD. Therefore, we herein report a novel method of sampling GCF from a mouse model of periodontitis, involving a series of easy steps. First, the original ligature used for induction of PD was removed, and a fresh ligature for sampling GCF was placed in the gingival crevice for 10min. Immediately afterwards, the volume of GCF collected in the sampling ligature was measured using a high precision weighing balance. The sampling ligature containing GCF was then immersed in a solution of PBS-Tween 20 and subjected to ELISA. This enabled us to monitor the volume of GCF and detect time-dependent changes in the expression of such cytokines as IL-1b, TNF-?, IL-6, RANKL, and OPG associated with the levels of alveolar bone loss, as reflected in GCF collected from a mouse model of PD. Therefore, this novel GCF sampling method can be used to measure various cytokines in GCF relative to the dynamic changes in periodontal bone loss induced in a mouse model of PD.

SUBMITTER: Matsuda S 

PROVIDER: S-EPMC5370161 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications


Using a mouse model of silk ligature-induced periodontal disease (PD), we report a novel method of sampling mouse gingival crevicular fluid (GCF) to evaluate the time-dependent secretion patterns of bone resorption-related cytokines. GCF is a serum transudate containing host-derived biomarkers which can represent cellular response in the periodontium. As such, human clinical evaluations of PD status rely on sampling this critical secretion. At the same time, a method of sampling GCF from mice is  ...[more]

Similar Datasets

2017-08-11 | GSE89081 | GEO
| S-EPMC5494293 | biostudies-literature
| PRJNA1165967 | ENA
| S-EPMC5089638 | biostudies-literature
| S-EPMC8453974 | biostudies-literature
| S-EPMC4359842 | biostudies-literature
| S-EPMC10824872 | biostudies-literature
| S-EPMC6301951 | biostudies-literature
| S-EPMC3162872 | biostudies-other
| S-EPMC6135659 | biostudies-literature