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Increased expression of protein kinase CK2? correlates with poor patient prognosis in epithelial ovarian cancer.

ABSTRACT: Epithelial ovarian cancer (EOC) is one of the deadly gynecological malignancies. The function of protein kinase CK2? (CK2?) in EOC is still unknown. Our study aimed to investigate the relationship between the protein expression of CK2? and the tumor progression, the prognosis of human EOC. In this study, we analyzed the expression levels of CK2? through Western blot, using EOC cell lines like A2780, HO8910, COV644, OVCAR3, SKOV3, and the primary normal ovarian surface epithelial (NOSE) cells. Furthermore, OVCAR3 and SKOV3 EOC cells were employed as a cellular model to study the role of CK2? on cell growth, migration, invasion, apoptosis, and cell cycle distribution. In addition, we investigated CK2? protein expression in tumor tissues from patients with EOC by immunohistochemistry and analyzed the association between CK2? expression and clinicopathologic parameters and prognosis of EOC patients. And we found that compared with NOSE cells, CK2? protein expression was increased in A2780, HO8910, OVCAR3, and SKOV3 ovarian cancer cell lines. Decreased CK2? expression suppressed OVCAR3 and SKOV3 cell growth and induced more apoptosis. CK2? knockdown using specific siRNAs inhibited migration and invasion ability of OVCAR3 and SKOV3 cells. In addition, high CK2? protein expression was found in 68.4% (80/117) of EOC patients. Increased CK2? expression of was significantly correlated with FIGO staging and peritoneal cytology. Patients with higher CK2? expression had a significantly poorer overall survival compared with those with lower CK2? expression. Multi-variate Cox regression analysis proved that increased CK2? expression was an independent prognostic marker for EOC. Taken together, our data displayed that CK2? may play a role in tumor aggressive behavior of EOC and could be used as a marker for predicting prognosis of EOC patient. High CK2? expression might predict poor patient survival.

SUBMITTER: Ma Z

PROVIDER: S-EPMC5371331 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Increased expression of protein kinase CK2α correlates with poor patient prognosis in epithelial ovarian cancer.

Ma Zebiao Z Wang Xiaojing X He Jiehua J Xia Jianchuan J Li Yanfang Y

PloS one 20170329 3

Epithelial ovarian cancer (EOC) is one of the deadly gynecological malignancies. The function of protein kinase CK2α (CK2α) in EOC is still unknown. Our study aimed to investigate the relationship between the protein expression of CK2α and the tumor progression, the prognosis of human EOC. In this study, we analyzed the expression levels of CK2α through Western blot, using EOC cell lines like A2780, HO8910, COV644, OVCAR3, SKOV3, and the primary normal ovarian surface epithelial (NOSE) cells. Fu ...[more]

PMID: 28355289

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Project description:Background: Insulin-like growth factor binding protein-7 (IGFBP7) contributes to multiple biological processes in various tumors. However, the role of IGFBP7 in gastric cancer (GC) is still undetermined. The study aims to explore the role of IGFBP7 in GC via an integrated bioinformatics analysis. Methods: IGFBP7 expression levels in GC and its normal gastric tissues were analyzed using multiple databases, including the Tumor Immune Estimation Resource (TIMER), Oncomine, The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases, as well as by our clinical gastric specimens. The methylation analysis was conducted with MEXPRESS, UALCAN and Xena online tools. The survival analysis was conducted using the Kaplan-Meier Plotter and Gene Expression Profiling Interactive Analysis (GEPIA) databases. Coexpressed genes of IGFBP7 were selected with the cBioPortal tool and enrichment analysis was conducted with the clusterProfiler package in R software. Gene set enrichment analysis (GSEA) was performed to explore the IGFBP7-related biological processes involved in GC. Correlations between IGFBP7 and immune cell infiltrates were analyzed using the TIMER database. Results: IGFBP7 expression was significantly upregulated in GC and correlated with stage, grade, tumor status and Helicobacter pylori infection. High IGFBP7 expression and low IGFBP7 methylation levels were significantly associated with short survival of patients with GC. Univariate and multivariate analyses revealed that IGFBP7 was an independent risk factor for GC. The coexpressed genes LHFPL6, SEPTIN4, HSPB2, LAYN and GGT5 predicted unfavorable outcomes of GC. Enrichment analysis showed that the coexpressed genes were involved in extracellular matrix (ECM)-related processes. GSEA indicated that IGFBP7 was positively related to ECM and inflammation-related pathways. TIMER analysis indicated that the mRNA level of IGFBP7 was strongly correlated with genes related to various infiltrating immune cells in GC, especially with gene markers of tumor associated macrophages (TAMs). Conclusions: Increased IGFBP7 expression correlates with poor prognosis and immune cell infiltration in GC, which might be a potential biomarker for the diagnosis of GC.

Dataset Information

Increased expression of protein kinase CK2? correlates with poor patient prognosis in epithelial ovarian cancer.

Publications

Increased expression of protein kinase CK2α correlates with poor patient prognosis in epithelial ovarian cancer.

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