Ontology highlight
ABSTRACT:
SUBMITTER: Demo G
PROVIDER: S-EPMC5378476 | biostudies-literature | 2017 Mar
REPOSITORIES: biostudies-literature
eLife 20170316
ArfA rescues ribosomes stalled on truncated mRNAs by recruiting release factor RF2, which normally binds stop codons to catalyze peptide release. We report two 3.2 Å resolution cryo-EM structures - determined from a single sample - of the 70S ribosome with ArfA•RF2 in the A site. In both states, the ArfA C-terminus occupies the mRNA tunnel downstream of the A site. One state contains a compact inactive RF2 conformation. Ordering of the ArfA N-terminus in the second state rearranges RF2 into an e ...[more]