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BRASSINOSTEROID INSENSITIVE2 negatively regulates cellulose synthesis in Arabidopsis by phosphorylating cellulose synthase 1.

ABSTRACT: The deposition of cellulose is a defining aspect of plant growth and development, but regulation of this process is poorly understood. Here, we demonstrate that the protein kinase BRASSINOSTEROID INSENSITIVE2 (BIN2), a key negative regulator of brassinosteroid (BR) signaling, can phosphorylate Arabidopsis cellulose synthase A1 (CESA1), a subunit of the primary cell wall cellulose synthase complex, and thereby negatively regulate cellulose biosynthesis. Accordingly, point mutations of the BIN2-mediated CESA1 phosphorylation site abolished BIN2-dependent regulation of cellulose synthase activity. Hence, we have uncovered a mechanism for how BR signaling can modulate cellulose synthesis in plants.

SUBMITTER: Sanchez-Rodriguez C

PROVIDER: S-EPMC5380027 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

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BRASSINOSTEROID INSENSITIVE2 negatively regulates cellulose synthesis in <i>Arabidopsis</i> by phosphorylating cellulose synthase 1.

Sánchez-Rodríguez Clara C Ketelaar KassaDee K Schneider Rene R Villalobos Jose A JA Somerville Chris R CR Persson Staffan S Wallace Ian S IS

Proceedings of the National Academy of Sciences of the United States of America 20170313 13

The deposition of cellulose is a defining aspect of plant growth and development, but regulation of this process is poorly understood. Here, we demonstrate that the protein kinase BRASSINOSTEROID INSENSITIVE2 (BIN2), a key negative regulator of brassinosteroid (BR) signaling, can phosphorylate <i>Arabidopsis</i> cellulose synthase A1 (CESA1), a subunit of the primary cell wall cellulose synthase complex, and thereby negatively regulate cellulose biosynthesis. Accordingly, point mutations of the ...[more]

PMID: 28289192

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