Dramatic improvement in genome assembly achieved using doubled-haploid genomes.
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ABSTRACT:
SUBMITTER: Zhang H
PROVIDER: S-EPMC5381364 | biostudies-literature | 2014 Oct
REPOSITORIES: biostudies-literature
Publications
Dramatic improvement in genome assembly achieved using doubled-haploid genomes.
Zhang Hong H Tan Engkong E Suzuki Yutaka Y Hirose Yusuke Y Kinoshita Shigeharu S Okano Hideyuki H Kudoh Jun J Shimizu Atsushi A Saito Kazuyoshi K Watabe Shugo S Asakawa Shuichi S
Scientific reports 20141027
Improvement in de novo assembly of large genomes is still to be desired. Here, we improved draft genome sequence quality by employing doubled-haploid individuals. We sequenced wildtype and doubled-haploid Takifugu rubripes genomes, under the same conditions, using the Illumina platform and assembled contigs with SOAPdenovo2. We observed 5.4-fold and 2.6-fold improvement in the sizes of the N50 contig and scaffold of doubled-haploid individuals, respectively, compared to the wildtype, indicating ...[more]
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