Unknown

Dataset Information

0

Badger macrophages fail to produce nitric oxide, a key anti-mycobacterial effector molecule.


ABSTRACT: The European badger is recognised as a wildlife reservoir for bovine tuberculosis (bTB); the control of which is complex, costly and controversial. Despite the importance of badgers in bTB and the well-documented role for macrophages as anti-mycobacterial effector cells, badger macrophage (bdM?) responses remain uncharacterised. Here, we demonstrate that bdM? fail to produce nitric oxide (NO) or upregulate inducible nitric oxide synthase (iNOS) mRNA following Toll-like receptor (TLR) agonist treatment. BdM? also failed to make NO after stimulation with recombinant badger interferon gamma (bdIFN?) or a combination of bdIFN? and lipopolysaccharide. Exposure of bdM? to TLR agonists and/or bdIFN? resulted in upregulated cytokine (IL1?, IL6, IL12 and TNF?) mRNA levels indicating that these critical pathways were otherwise intact. Although stimulation with most TLR agonists resulted in strong cytokine mRNA responses, weaker responses were evident after exposure to TLR9 agonists, potentially due to very low expression of TLR9 in bdM?. Both NO and TLR9 are important elements of innate immunity to mycobacteria, and these features of bdM? biology would impair their capacity to resist bTB infection. These findings have significant implications for the development of bTB management strategies, and support the use of vaccination to reduce bTB infection in badgers.

SUBMITTER: Bilham K 

PROVIDER: S-EPMC5382539 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications

Badger macrophages fail to produce nitric oxide, a key anti-mycobacterial effector molecule.

Bilham Kirstin K   Boyd Amy C AC   Preston Stephen G SG   Buesching Christina D CD   Newman Chris C   Macdonald David W DW   Smith Adrian L AL  

Scientific reports 20170406


The European badger is recognised as a wildlife reservoir for bovine tuberculosis (bTB); the control of which is complex, costly and controversial. Despite the importance of badgers in bTB and the well-documented role for macrophages as anti-mycobacterial effector cells, badger macrophage (bdMφ) responses remain uncharacterised. Here, we demonstrate that bdMφ fail to produce nitric oxide (NO) or upregulate inducible nitric oxide synthase (iNOS) mRNA following Toll-like receptor (TLR) agonist tre  ...[more]

Similar Datasets

| S-EPMC5430514 | biostudies-literature
| S-EPMC8678072 | biostudies-literature
| S-EPMC4812255 | biostudies-other
| S-EPMC3754229 | biostudies-literature
| S-EPMC3995984 | biostudies-literature
| S-EPMC2134953 | biostudies-literature
| S-EPMC6963498 | biostudies-literature
| S-EPMC5697141 | biostudies-literature
| S-EPMC3538947 | biostudies-literature
| S-EPMC10790689 | biostudies-literature