Dataset Information

Pulmonary Delivery of Virosome-Bound Antigen Enhances Antigen-Specific CD4⁺ T Cell Proliferation Compared to Liposome-Bound or Soluble Antigen.

ABSTRACT: Pulmonary administration of biomimetic nanoparticles loaded with antigen may represent an effective strategy to directly modulate adaptive immune responses in the respiratory tract. Depending on the design, virosomes may not only serve as biomimetic antigen carriers but are also endowed with intrinsic immune-stimulatory properties. We designed fluorescently labeled influenza-derived virosomes and liposome controls coupled to the model antigen ovalbumin to investigate uptake, phenotype changes, and antigen processing by antigen-presenting cells exposed to such particles in different respiratory tract compartments. Both virosomes and liposomes were captured by pulmonary macrophages and dendritic cells alike and induced activation in particle-bearing cells by upregulation of costimulatory markers such as CD40, CD80, CD86, PD-L1, PD-L2, and ICOS-L. Though antigen processing and accumulation of both coupled and soluble antigen was similar between virosomes and liposomes, only ovalbumin-coupled virosomes generated a strong antigen-specific CD4⁺ T cell proliferation. Pulmonary administrated antigen-coupled virosomes therefore effectively induced adaptive immune responses and may be utilized in novel preventive or therapeutic approaches in the respiratory tract.

SUBMITTER: Blom RAM

PROVIDER: S-EPMC5383731 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Pulmonary Delivery of Virosome-Bound Antigen Enhances Antigen-Specific CD4<sup>+</sup> T Cell Proliferation Compared to Liposome-Bound or Soluble Antigen.

Blom Rebecca A M RAM Amacker Mario M van Dijk R Maarten RM Moser Christian C Stumbles Philip A PA Blank Fabian F von Garnier Christophe C

Frontiers in immunology 20170407

Pulmonary administration of biomimetic nanoparticles loaded with antigen may represent an effective strategy to directly modulate adaptive immune responses in the respiratory tract. Depending on the design, virosomes may not only serve as biomimetic antigen carriers but are also endowed with intrinsic immune-stimulatory properties. We designed fluorescently labeled influenza-derived virosomes and liposome controls coupled to the model antigen ovalbumin to investigate uptake, phenotype changes, a ...[more]

PMID: 28439267

Similar Datasets

Project description:Alzheimer’s disease (AD) is the most common cause of late-life dementia characterized by progressive neurodegeneration and brain deposition of amyloid-β (Aβ) and phosphorylated tau. The APOE ε2 encoding apolipoprotein E (APOE2) is a protective allele against AD among the three genotypes (APOE ε2, ε3, ε4), while APOE4 is the strongest genetic factor substantially increasing AD risk. APOE regulates brain lipid homeostasis and maintaining synaptic plasticity and neuronal function, where APOE2 has a superior function compared to APOE3 and APOE4. Gene therapy that increases APOE2 levels in the brain is, therefore, a promising therapeutic strategy for AD treatment. We previously reported that PEGylated liposomes conjugated with transferrin and a cell-penetrating peptide Penetratin sufficiently deliver chitosan-APOE2 cDNA plasmid complex into the brain of wild-type mice. Here, we investigated how brain-targeting liposome-based APOE2 gene delivery influences Aβ)-related pathologies in amyloid model AppNL-G-F knockin mice at 12-month-old. We found a trend of reductions of insoluble Aβ levels in the mouse cortices 1 month after APOE2 gene therapy. Furthermore, in the AppNL-G-F knockin mice that received the APOE2 gene therapy, brain transcriptome analysis through RNA-sequencing identified the upregulation of genes/pathways related to neuronal development. This was supported by increases of Dlg4 and Syp mRNAs coding synaptic proteins in the experimental group. On the other hand, we found that APOE2 gene delivery increased soluble Aβ levels, including oligomers, as well as exacerbated neurite dystrophy and decreased synaptophysin. Together, our results suggest that brain-targeting liposome-based APOE2 gene therapy is potentially beneficial for synaptic formation at the transcriptional level. Forced APOE2 expressions, however, may exacerbate Aβ toxicity by increasing the dissociation of Aβ oligomers from aggregates in the presence of considerable amyloid burden.

Dataset Information

Pulmonary Delivery of Virosome-Bound Antigen Enhances Antigen-Specific CD4⁺ T Cell Proliferation Compared to Liposome-Bound or Soluble Antigen.

Publications

Pulmonary Delivery of Virosome-Bound Antigen Enhances Antigen-Specific CD4<sup>+</sup> T Cell Proliferation Compared to Liposome-Bound or Soluble Antigen.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Pulmonary Delivery of Virosome-Bound Antigen Enhances Antigen-Specific CD4+ T Cell Proliferation Compared to Liposome-Bound or Soluble Antigen.

Publications

Pulmonary Delivery of Virosome-Bound Antigen Enhances Antigen-Specific CD4<sup>+</sup> T Cell Proliferation Compared to Liposome-Bound or Soluble Antigen.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Pulmonary Delivery of Virosome-Bound Antigen Enhances Antigen-Specific CD4⁺ T Cell Proliferation Compared to Liposome-Bound or Soluble Antigen.