Intestinal microbiome in children with severe and complicated acute viral gastroenteritis.

ABSTRACT: The aim of the present study was to evaluate the microbiota of children with severe or complicated acute viral gastroenteritis (AGE). To that end, next-generation sequencing (NGS) technology was used to sequence the 16S ribosomal RNA (16S rRNA) gene in 20 hospitalized pediatric patients with severe or complicated AGE and a further 20 otherwise healthy children; the fecal microbiome was then assessed. Comparative metagenomics data were analyzed by a Wilcoxon rank-sum test and hierarchical clustering analysis of bacterial reads. The statistical analyses showed a significantly decreased Shannon diversity index (entropy score) of the intestinal microbiota in patients with severe AGE compared with normal controls (P?=?0.017) and patients with mild-to-moderate AGE (P?=?0.011). The intestinal microbiota score of the 5 patients with rotavirus AGE was significantly lower than that of those with norovirus infection (P?=?0.048). Greater richness in Campylobacteraceae (P?=?0.0003), Neisseriaceae (P?=?0.0115), Methylobacteriaceae (P?=?0.0004), Sphingomonadaceae (P?=?0.0221), and Enterobacteriaceae (P?=?0.0451) was found in patients with complicated AGE compared with normal controls. The data suggest a significant reduction in intestinal microbial diversity in patients with severe AGE, particularly those with rotavirus infection.

SUBMITTER: Chen SY 

PROVIDER: S-EPMC5387401 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature