Dataset Information

Hsa-miR-19a is associated with lymph metastasis and mediates the TNF-? induced epithelial-to-mesenchymal transition in colorectal cancer.

ABSTRACT: Lymph node metastasis is an important factor determining the outcome of colorectal cancer. Although epithelial-to-mesenchymal transition (EMT), TNF-? and microRNA (miRNA) have been found to play important roles in lymph node metastasis, the underlying molecular mechanism remains unclear. Here we reported that high expression of microRNA-19a (miR-19a) was associated with lymph node metastasis and played an important role in TNF-?-induced EMT in colorectal cancer (CRC) cells. We analyzed miR-19a expression in surgical tissue specimens from 11 CRC patients and 275 formalin-fixed, paraffin-embedded CRC patients. We found that miR-19a was up-regulated in CRC tissues and high expression of miR-19a was significantly associated with lymph node metastasis. We further analyzed miR-19a lymph node metastasis signature in an external validation cohort of 311 CRC cases of the TCGA. MiR-19a was found to be significantly associated with lymph node metastasis in rectal cancer. In vitro, we showed that overexpression of miR-19a in human CRC cell lines promoted cell invasion and EMT. Furthermore, miR-19a was up-regulated by TNF-? and miR-19a was required for TNF-?-induced EMT and metastasis in CRC cells. Collectively, miR-19a played an important role in mediating EMT and metastatic behavior in CRC. It may serve as a potential marker of lymph node metastasis.

SUBMITTER: Huang L

PROVIDER: S-EPMC5388049 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

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Hsa-miR-19a is associated with lymph metastasis and mediates the TNF-α induced epithelial-to-mesenchymal transition in colorectal cancer.

Huang Lanlan L Wang Xiaoyan X Wen Chuangyu C Yang Xiangling X Song Minmin M Chen Junxiong J Wang Chenliang C Zhang Bo B Wang Lei L Iwamoto Aikichi A Wang Jianping J Liu Huanliang H

Scientific reports 20150825

Lymph node metastasis is an important factor determining the outcome of colorectal cancer. Although epithelial-to-mesenchymal transition (EMT), TNF-α and microRNA (miRNA) have been found to play important roles in lymph node metastasis, the underlying molecular mechanism remains unclear. Here we reported that high expression of microRNA-19a (miR-19a) was associated with lymph node metastasis and played an important role in TNF-α-induced EMT in colorectal cancer (CRC) cells. We analyzed miR-19a e ...[more]

PMID: 26302825

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Dataset Information

Hsa-miR-19a is associated with lymph metastasis and mediates the TNF-? induced epithelial-to-mesenchymal transition in colorectal cancer.

Publications

Hsa-miR-19a is associated with lymph metastasis and mediates the TNF-α induced epithelial-to-mesenchymal transition in colorectal cancer.

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