Ontology highlight
ABSTRACT:
SUBMITTER: Trubetskoy VS
PROVIDER: S-EPMC5388421 | biostudies-literature | 2017 Feb
REPOSITORIES: biostudies-literature
Trubetskoy Vladimir S VS Griffin Jacob B JB Nicholas Anthony L AL Nord Eric M EM Xu Zhao Z Peterson Ryan M RM Wooddell Christine I CI Rozema David B DB Wakefield Darren H DH Lewis David L DL Kanner Steven B SB
Nucleic acids research 20170201 3
The RNA interference (RNAi)-based therapeutic ARC-520 for chronic hepatitis B virus (HBV) infection consists of a melittin-derived peptide conjugated to N-acetylgalactosamine for hepatocyte targeting and endosomal escape, and cholesterol-conjugated RNAi triggers, which together result in HBV gene silencing. To characterize the kinetics of RNAi trigger delivery and 5΄-phosphorylation of guide strands correlating with gene knockdown, we employed a peptide-nucleic acid (PNA) hybridization assay. A ...[more]