Ontology highlight
ABSTRACT:
SUBMITTER: Mentegari E
PROVIDER: S-EPMC5389505 | biostudies-literature | 2017 Mar
REPOSITORIES: biostudies-literature
Mentegari Elisa E Crespan Emmanuele E Bavagnoli Laura L Kissova Miroslava M Bertoletti Federica F Sabbioneda Simone S Imhof Ralph R Sturla Shana J SJ Nilforoushan Arman A Hübscher Ulrich U van Loon Barbara B Maga Giovanni G
Nucleic acids research 20170301 5
Ribonucleotides (rNs) incorporated in the genome by DNA polymerases (Pols) are removed by RNase H2. Cytidine and guanosine preferentially accumulate over the other rNs. Here we show that human Pol η can incorporate cytidine monophosphate (rCMP) opposite guanine, 8-oxo-7,8-dihydroguanine, 8-methyl-2΄-deoxyguanosine and a cisplatin intrastrand guanine crosslink (cis-PtGG), while it cannot bypass a 3-methylcytidine or an abasic site with rNs as substrates. Pol η is also capable of synthesizing poly ...[more]