Ontology highlight
ABSTRACT:
SUBMITTER: Musharova O
PROVIDER: S-EPMC5389516 | biostudies-literature | 2017 Apr
REPOSITORIES: biostudies-literature
Musharova Olga O Klimuk Evgeny E Datsenko Kirill A KA Metlitskaya Anastasia A Logacheva Maria M Semenova Ekaterina E Severinov Konstantin K Savitskaya Ekaterina E
Nucleic acids research 20170401 6
During primed CRISPR adaptation spacers are preferentially selected from DNA recognized by CRISPR interference machinery, which in the case of Type I CRISPR-Cas systems consists of CRISPR RNA (crRNA) bound effector Cascade complex that locates complementary targets, and Cas3 executor nuclease/helicase. A complex of Cas1 and Cas2 proteins is capable of inserting new spacers in the CRISPR array. Here, we show that in Escherichia coli cells undergoing primed adaptation, spacer-sized fragments of fo ...[more]