Managing the sequence-specificity of antisense oligonucleotides in drug discovery.

ABSTRACT: All drugs perturb the expression of many genes in the cells that are exposed to them. These gene expression changes can be divided into effects resulting from engaging the intended target and effects resulting from engaging unintended targets. For antisense oligonucleotides, developments in bioinformatics algorithms, and the quality of sequence databases, allow oligonucleotide sequences to be analyzed computationally, in terms of the predictability of their interactions with intended and unintended RNA targets. Applying these tools enables selection of sequence-specific oligonucleotides where no- or only few unintended RNA targets are expected. To evaluate oligonucleotide sequence-specificity experimentally, we recommend a transcriptomics protocol where two or more oligonucleotides targeti

SUBMITTER: Hagedorn PH 

PROVIDER: S-EPMC5389529 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature