Dataset Information

Mucosal IgA and IFN-?+ CD8 T cell immunity are important in the efficacy of live Salmonella enteria serovar Choleraesuis vaccines.

ABSTRACT: Salmonellosis, a disease caused by non-typhoidal Salmonella strains which can be transmitted from swine to humans, is one of the leading public health problems around the world. Paratyphoid of swine is controlled by vaccinating swine with Salmonella enterica serovar Choleraesuis (S. Choleraesuis) live vaccine strain C500 in China. Although the vaccine has good prophylactic efficacy, the mechanism of immunogenicity is unclear. Using a C500-derived paratyphoid thermo-stable live vaccine (PTSL vaccine), we demonstrated that the PTSL vaccine induces strong primary and memory immune responses in piglets. Mucosal IgA and IFN-?+/CD8+ T cells induced by the PTSL vaccine play key roles in the protection of the host from Salmonella infection. Our findings have important implications on the development of new and improved vaccines against salmonellosis and using live-attenuated Salmonella as vaccine carriers.

SUBMITTER: Zhu L

PROVIDER: S-EPMC5390296 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature

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Mucosal IgA and IFN-γ<sup>+</sup> CD8 T cell immunity are important in the efficacy of live Salmonella enteria serovar Choleraesuis vaccines.

Zhu Liangquan L Zhao Xinxin X Yin Qing Q Liu Xianyong X Chen Xiang X Huang Chunjuan C Suo Xun X

Scientific reports 20170413

Salmonellosis, a disease caused by non-typhoidal Salmonella strains which can be transmitted from swine to humans, is one of the leading public health problems around the world. Paratyphoid of swine is controlled by vaccinating swine with Salmonella enterica serovar Choleraesuis (S. Choleraesuis) live vaccine strain C500 in China. Although the vaccine has good prophylactic efficacy, the mechanism of immunogenicity is unclear. Using a C500-derived paratyphoid thermo-stable live vaccine (PTSL vacc ...[more]

PMID: 28406162

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Project description:Salmonella enterica subsp. enterica serovar Choleraesuis is a highly invasive pathogen of swine that frequently causes serious outbreaks, in particular in Asia, and can also cause severe invasive disease in humans. In this study, 21 S. Choleraesuis isolates, detected from 21 patients with diarrhea in China between 2010 and 2011, were found to include 19 H2S-negative S. Choleraesuis isolates and two H2S-positive isolates. This is the first report of H2S-negative S. Choleraesuis isolated from humans. The majority of H2S-negative isolates exhibited high resistance to ampicillin, chloramphenicol, gentamicin, tetracycline, ticarcillin, and trimethoprim-sulfamethoxazole, but only six isolates were resistant to norfloxacin. In contrast, all of the isolates were sensitive to cephalosporins. Fifteen isolates were found to be multidrug resistant. In norfloxacin-resistant isolates, we detected mutations in the gyrA and parC genes and identified two new mutations in the parC gene. Pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and clustered regularly interspaced short palindromic repeat (CRISPR) analysis were employed to investigate the genetic relatedness of H2S-negative and H2S-positive S. Choleraesuis isolates. PFGE revealed two groups, with all 19 H2S-negative S. Choleraesuis isolates belonging to Group I and H2S-positive isolates belonging to Group II. By MLST analysis, the H2S-negative isolates were all found to belong to ST68 and H2S-positive isolates belong to ST145. By CRISPR analysis, no significant differences in CRISPR 1 were detected; however, one H2S-negative isolate was found to contain three new spacers in CRISPR 2. All 19 H2S-negative isolates also possessed a frame-shift mutation at position 760 of phsA gene compared with H2S-positive isolates, which may be responsible for the H2S-negative phenotype. Moreover, the 19 H2S-negative isolates have similar PFGE patterns and same mutation site in the phsA gene, these results indicated that these H2S-negative isolates may have been prevalent in China. These findings suggested that surveillance should be increased of H2S-negative S. Choleraesuis in China.

Dataset Information

Mucosal IgA and IFN-?+ CD8 T cell immunity are important in the efficacy of live Salmonella enteria serovar Choleraesuis vaccines.

Publications

Mucosal IgA and IFN-γ<sup>+</sup> CD8 T cell immunity are important in the efficacy of live Salmonella enteria serovar Choleraesuis vaccines.

Similar Datasets

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