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Dual functionality of cis-regulatory elements as developmental enhancers and Polycomb response elements.


ABSTRACT: Developmental gene expression is tightly regulated through enhancer elements, which initiate dynamic spatio-temporal expression, and Polycomb response elements (PREs), which maintain stable gene silencing. These two cis-regulatory functions are thought to operate through distinct dedicated elements. By examining the occupancy of the Drosophila pleiohomeotic repressive complex (PhoRC) during embryogenesis, we revealed extensive co-occupancy at developmental enhancers. Using an established in vivo assay for PRE activity, we demonstrated that a subset of characterized developmental enhancers can function as PREs, silencing transcription in a Polycomb-dependent manner. Conversely, some classic Drosophila PREs can function as developmental enhancers in vivo, activating spatio-temporal expression. This study therefore uncovers elements with dual function: activating transcription in some cells (enhancers) while stably maintaining transcriptional silencing in others (PREs). Given that enhancers initiate spatio-temporal gene expression, reuse of the same elements by the Polycomb group (PcG) system may help fine-tune gene expression and ensure the timely maintenance of cell identities.

SUBMITTER: Erceg J 

PROVIDER: S-EPMC5393054 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

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Dual functionality of <i>cis</i>-regulatory elements as developmental enhancers and Polycomb response elements.

Erceg Jelena J   Pakozdi Tibor T   Marco-Ferreres Raquel R   Ghavi-Helm Yad Y   Girardot Charles C   Bracken Adrian P AP   Furlong Eileen E M EE  

Genes & development 20170301 6


Developmental gene expression is tightly regulated through enhancer elements, which initiate dynamic spatio-temporal expression, and Polycomb response elements (PREs), which maintain stable gene silencing. These two <i>cis</i>-regulatory functions are thought to operate through distinct dedicated elements. By examining the occupancy of the <i>Drosophila</i> pleiohomeotic repressive complex (PhoRC) during embryogenesis, we revealed extensive co-occupancy at developmental enhancers. Using an estab  ...[more]

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