Ontology highlight
ABSTRACT:
SUBMITTER: Ito Y
PROVIDER: S-EPMC5393199 | biostudies-literature | 2017 Apr
REPOSITORIES: biostudies-literature
Ito Yoshiaki Y Inoue Atsushi A Seers Timothy T Hato Yukari Y Igarashi Arisa A Toyama Tatsuya T Taganov Konstantin D KD Boldin Mark P MP Asahara Hiroshi H
Proceedings of the National Academy of Sciences of the United States of America 20170329 15
miRNAs play critical roles in various biological processes by targeting specific mRNAs. Current approaches to identifying miRNA targets are insufficient for elucidation of a miRNA regulatory network. Here, we created a cell-based screening system using a luciferase reporter library composed of 4,891 full-length cDNAs, each of which was integrated into the 3' UTR of a luciferase gene. Using this reporter library system, we conducted a screening for targets of miR-34a, a tumor-suppressor miRNA. We ...[more]