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Optical visualization and quantification of enzyme activity using dynamic droplet lenses.

ABSTRACT: In this paper, we describe an approach to measuring enzyme activity based on the reconfiguration of complex emulsions. Changes in the morphology of these complex emulsions, driven by enzyme-responsive surfactants, modulate the transmission of light through a sample. Through this method we demonstrate how simple photodetector measurements may be used to monitor enzyme kinetics. This approach is validated by quantitative measurements of enzyme activity for three different classes of enzymes (amylase, lipase, and sulfatase), relying on two distinct mechanisms for coupling droplet morphology to enzyme activity (host-guest interactions with uncaging and molecular cleavage).

SUBMITTER: Zarzar LD

PROVIDER: S-EPMC5393242 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature

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Optical visualization and quantification of enzyme activity using dynamic droplet lenses.

Zarzar Lauren D LD Kalow Julia A JA He Xinping X Walish Joseph J JJ Swager Timothy M TM

Proceedings of the National Academy of Sciences of the United States of America 20170327 15

In this paper, we describe an approach to measuring enzyme activity based on the reconfiguration of complex emulsions. Changes in the morphology of these complex emulsions, driven by enzyme-responsive surfactants, modulate the transmission of light through a sample. Through this method we demonstrate how simple photodetector measurements may be used to monitor enzyme kinetics. This approach is validated by quantitative measurements of enzyme activity for three different classes of enzymes (amyla ...[more]

PMID: 28348236

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