Ontology highlight
ABSTRACT:
SUBMITTER: Gupta S
PROVIDER: S-EPMC5394351 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
Gupta Shivani S Taylor Tracy T Patterson Aileen A Liang Binhua B Bullard Jared J Sandstrom Paul P Van Domselaar Gary G Ji Hezhao H
BioMed research international 20170403
The prevalence of drug resistance (DR) mutations in people with HIV-1 infection, particularly those with low-level viremia (LLV), supports the need to improve the sensitivity of amplification methods for HIV DR genotyping in order to optimize antiretroviral regimen and facilitate HIV-1 DR surveillance and relevant research. Here we report on a fully validated PCR-based protocol that achieves consistent amplification of the protease (PR) and reverse transcriptase (RT) regions of HIV-1 <i>pol</i> ...[more]