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Simultaneous impedance spectroscopy and fluorescence microscopy for the real-time monitoring of the response of cells to drugs.

ABSTRACT: A dual fluorescence microscopy and electrochemical strategy to investigate how cell-surface interactions influence the cellular responses to cues for the cell-based biosensing of drug efficacy is reported herein. The combined method can be used to not only monitor the importance of controlling the cellular adhesive environment on the cell response to drugs but it also provides biological information on the timescales of downstream outside-in signaling from soluble cues. As an example of the use of the combined method, we show how adhesive cues influence the signalling responses of cells to soluble cues. G-protein-coupled receptors were used as the target for the soluble cues. The changes in cell adhesion, cell morphology and Ca²⁺ flux induced by soluble histamine were simultaneously monitored as a function of the spacing of the adhesive ligand RGD on the interdigitated indium tin oxide electrodes. The simultaneous measurements revealed that the timescales of histamine-induced Ca²⁺ mobilization and the decrease in cell-cell adhesions are correlated. Furthermore, cells on the surfaces with an RGD spacing of 31 nm were shown to display a faster release of Ca²⁺ and change in cell adhesion upon histamine stimulation compared to cells on other surfaces.

SUBMITTER: Parviz M

PROVIDER: S-EPMC5396555 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

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Simultaneous impedance spectroscopy and fluorescence microscopy for the real-time monitoring of the response of cells to drugs.

Parviz M M Gaus K K Gooding J J JJ

Chemical science 20170103 3

A dual fluorescence microscopy and electrochemical strategy to investigate how cell-surface interactions influence the cellular responses to cues for the cell-based biosensing of drug efficacy is reported herein. The combined method can be used to not only monitor the importance of controlling the cellular adhesive environment on the cell response to drugs but it also provides biological information on the timescales of downstream outside-in signaling from soluble cues. As an example of the use ...[more]

PMID: 28451304

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Project description:Real-time monitoring of the brain is potentially valuable for performance monitoring, communication, training or rehabilitation. In natural situations, the brain performs a complex mix of various sensory, motor or cognitive functions. Thus, real-time brain monitoring would be most valuable if (a) it could decode information from multiple brain systems simultaneously, and (b) this decoding of each brain system were robust to variations in the activity of other (unrelated) brain systems. Previous studies showed that it is possible to decode some information from different brain systems in retrospect and/or in isolation. In our study, we set out to determine whether it is possible to simultaneously decode important information about a user from different brain systems in real time, and to evaluate the impact of concurrent activity in different brain systems on decoding performance.We study these questions using electrocorticographic signals recorded in humans. We first document procedures for generating stable decoding models given little training data, and then report their use for offline and for real-time decoding from 12 subjects (six for offline parameter optimization, six for online experimentation). The subjects engage in tasks that involve movement intention, movement execution and auditory functions, separately, and then simultaneously. Main Results: Our real-time results demonstrate that our system can identify intention and movement periods in single trials with an accuracy of 80.4% and 86.8%, respectively (where 50% would be expected by chance). Simultaneously, the decoding of the power envelope of an auditory stimulus resulted in an average correlation coefficient of 0.37 between the actual and decoded power envelopes. These decoders were trained separately and executed simultaneously in real time.This study yielded the first demonstration that it is possible to decode simultaneously the functional activity of multiple independent brain systems. Our comparison of univariate and multivariate decoding strategies, and our analysis of the influence of their decoding parameters, provides benchmarks and guidelines for future research on this topic.

Dataset Information

Simultaneous impedance spectroscopy and fluorescence microscopy for the real-time monitoring of the response of cells to drugs.

Publications

Simultaneous impedance spectroscopy and fluorescence microscopy for the real-time monitoring of the response of cells to drugs.

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