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Challenges in enumeration of CTCs in breast cancer using techniques independent of cytokeratin expression.

ABSTRACT:

Introduction

Given the current postulated plasticity between epithelial and mesenchymal states of migratory cancer cells the detection of non-epithelial CTCs is an important scientific and clinical goal.

Methods

We used the filtration-based ISET technology to enrich circulating tumour cells (CTCs) in early breast cancer blood samples and identify them using a morphology-based immunocytochemistry (ICC) approach.

Results

We found greater numbers of putative CTCs by this approach than by the cytokeratin-based CellSearch technology, but a high number of CTC false positives were identified in healthy volunteer samples which were not reduced in successive blood draws. Preliminary work using an oestrogen receptor (ER)-based multiplex ICC method in metastatic breast cancer ISET samples indicated a low number of ER+ CTCs even at this advanced stage.

Conclusions

This work highlights the challenges in enumerating CTCs without conventional epithelial markers.

SUBMITTER: Castle J

PROVIDER: S-EPMC5397021 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Challenges in enumeration of CTCs in breast cancer using techniques independent of cytokeratin expression.

Castle John J Morris Karen K Pritchard Susan S Kirwan Cliona C CC

PloS one 20170419 4

<h4>Introduction</h4>Given the current postulated plasticity between epithelial and mesenchymal states of migratory cancer cells the detection of non-epithelial CTCs is an important scientific and clinical goal.<h4>Methods</h4>We used the filtration-based ISET technology to enrich circulating tumour cells (CTCs) in early breast cancer blood samples and identify them using a morphology-based immunocytochemistry (ICC) approach.<h4>Results</h4>We found greater numbers of putative CTCs by this appro ...[more]

PMID: 28422972

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