Human Bub1 protects centromeric sister-chromatid cohesion through Shugoshin during mitosis.
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ABSTRACT:
SUBMITTER: Tang Z
PROVIDER: S-EPMC539817 | biostudies-literature | 2004 Dec
REPOSITORIES: biostudies-literature
Publications
Human Bub1 protects centromeric sister-chromatid cohesion through Shugoshin during mitosis.
Tang Zhanyun Z Sun Yuxiao Y Harley Sara E SE Zou Hui H Yu Hongtao H
Proceedings of the National Academy of Sciences of the United States of America 20041216 52
Sister chromatids in mammalian cells remain attached mostly at their centromeres at metaphase because of the loss of cohesion along chromosome arms in prophase. Here, we report that Bub1 retains centromeric cohesion in mitosis of human cells. Depletion of Bub1 or Shugoshin (Sgo1) in HeLa cells by RNA interference causes massive missegregation of sister chromatids that originates at centromeres. Surprisingly, loss of chromatid cohesion in Bub1 and Sgo1 RNA-interference cells does not appear to re ...[more]
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