Ontology highlight
ABSTRACT:
SUBMITTER: Nehme R
PROVIDER: S-EPMC5398546 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
Nehmé Rony R Carpenter Byron B Singhal Ankita A Strege Annette A Edwards Patricia C PC White Courtney F CF Du Haijuan H Grisshammer Reinhard R Tate Christopher G CG
PloS one 20170420 4
Mini-G proteins are the engineered GTPase domains of Gα subunits. They couple to GPCRs and recapitulate the increase in agonist affinity observed upon coupling of a native heterotrimeric G protein. Given the small size and stability of mini-G proteins, and their ease of expression and purification, they are ideal for biophysical studies of GPCRs in their fully active state. The first mini-G protein developed was mini-Gs. Here we extend the family of mini-G proteins to include mini-Golf, mini-Gi1 ...[more]