Ontology highlight
ABSTRACT:
SUBMITTER: Miyamoto K
PROVIDER: S-EPMC5399555 | biostudies-literature | 2017 Apr
REPOSITORIES: biostudies-literature
Miyamoto Kei K Tajima Yosuke Y Yoshida Koki K Oikawa Mami M Azuma Rika R Allen George E GE Tsujikawa Tomomi T Tsukaguchi Tomomasa T Bradshaw Charles R CR Jullien Jerome J Yamagata Kazuo K Matsumoto Kazuya K Anzai Masayuki M Imai Hiroshi H Gurdon John B JB Yamada Masayasu M
Biology open 20170415 4
Animal cloning has been achieved in many species by transplanting differentiated cell nuclei to unfertilized oocytes. However, the low efficiencies of cloning have remained an unresolved issue. Here we find that the combination of two small molecules, trichostatin A (TSA) and vitamin C (VC), under culture condition with bovine serum albumin deionized by ion-exchange resins, dramatically improves the cloning efficiency in mice and 15% of cloned embryos develop to term by means of somatic cell nuc ...[more]