Ontology highlight
ABSTRACT:
SUBMITTER: Azmi IF
PROVIDER: S-EPMC5400510 | biostudies-literature | 2017 Mar
REPOSITORIES: biostudies-literature
Azmi Ishara F IF Watanabe Shinya S Maloney Michael F MF Kang Sukhyun S Belsky Jason A JA MacAlpine David M DM Peterson Craig L CL Bell Stephen P SP
eLife 20170321
Eukaryotic replication origin licensing, activation and timing are influenced by chromatin but a mechanistic understanding is lacking. Using reconstituted nucleosomal DNA replication assays, we assessed the impact of nucleosomes on replication initiation. To generate distinct nucleosomal landscapes, different chromatin-remodeling enzymes (CREs) were used to remodel nucleosomes on origin-DNA templates. Nucleosomal organization influenced two steps of replication initiation: origin licensing and h ...[more]