Ontology highlight
ABSTRACT:
SUBMITTER: Rasmussen TB
PROVIDER: S-EPMC540114 | biostudies-literature | 2005 Jan
REPOSITORIES: biostudies-literature
Rasmussen Thomas B TB Uttenthal Ase A Fernández Jovita J Storgaard Torben T
Journal of clinical microbiology 20050101 1
In order to establish a rapid and reliable system for the detection of vesicular stomatitis virus (VSV), we developed a quantitative reverse transcription-PCR assay for the detection, quantification, and differentiation of the major serotypes, VSV Indiana and VSV New Jersey, using a closed-tube multiplex format. The detection system is based on the recently invented primer-probe energy transfer (PriProET) system. A region of the gene encoding the RNA-dependent RNA polymerase was amplified by usi ...[more]