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Detection of human influenza A viruses by loop-mediated isothermal amplification.


ABSTRACT: Here we describe the use of the loop-mediated isothermal amplification (LAMP) method to detect human influenza viruses (H1 to H3). Our results were correlated 100% with results deduced from routine clinical diagnostic tests. In addition, we also developed a LAMP assay specific for human beta-actin cDNA as a quality control test.

SUBMITTER: Poon LL 

PROVIDER: S-EPMC540134 | biostudies-literature | 2005 Jan

REPOSITORIES: biostudies-literature

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Detection of human influenza A viruses by loop-mediated isothermal amplification.

Poon Leo L M LL   Leung Cynthia S W CS   Chan Kwok H KH   Lee Jack H C JH   Yuen Kwok Y KY   Guan Yi Y   Peiris Joseph S M JS  

Journal of clinical microbiology 20050101 1


Here we describe the use of the loop-mediated isothermal amplification (LAMP) method to detect human influenza viruses (H1 to H3). Our results were correlated 100% with results deduced from routine clinical diagnostic tests. In addition, we also developed a LAMP assay specific for human beta-actin cDNA as a quality control test. ...[more]

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