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Molecular characterization of an Akabane virus isolate from West Java, Indonesia.


ABSTRACT: We isolated an arbovirus from bovine blood in Indonesia. The arbovirus was obtained from the plasma of a cow showing no clinical symptoms in West Java in February 2014, and was identified as Akabane virus (AKAV) by AKAV-specific RT-PCR and subsequent sequence analysis. Phylogenetic analysis based on partial S segment indicated the AKAV isolate, WJ-1SA/P/2014, was most closely related with two isolates from Israel and Turkey reported in 2001 and 2015, respectively, and that WJ-1SA/P/2014 isolate belongs to AKAV genogroup Ib. This is the first isolation of AKAV from Indonesia.

SUBMITTER: Purnomo Edi S 

PROVIDER: S-EPMC5402201 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature

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Molecular characterization of an Akabane virus isolate from West Java, Indonesia.

Purnomo Edi Suryo S   Ibrahim Afif A   Sukoco Rinto R   Bunali Lukman L   Taguchi Masaji M   Kato Tomoko T   Yanase Tohru T   Shirafuji Hiroaki H  

The Journal of veterinary medical science 20170308 4


We isolated an arbovirus from bovine blood in Indonesia. The arbovirus was obtained from the plasma of a cow showing no clinical symptoms in West Java in February 2014, and was identified as Akabane virus (AKAV) by AKAV-specific RT-PCR and subsequent sequence analysis. Phylogenetic analysis based on partial S segment indicated the AKAV isolate, WJ-1SA/P/2014, was most closely related with two isolates from Israel and Turkey reported in 2001 and 2015, respectively, and that WJ-1SA/P/2014 isolate  ...[more]

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