Ontology highlight
ABSTRACT:
SUBMITTER: Peracchi A
PROVIDER: S-EPMC5402446 | biostudies-literature | 2017 Apr
REPOSITORIES: biostudies-literature
Peracchi Alessio A Veiga-da-Cunha Maria M Kuhara Tomiko T Ellens Kenneth W KW Paczia Nicole N Stroobant Vincent V Seliga Agnieszka K AK Marlaire Simon S Jaisson Stephane S Bommer Guido T GT Sun Jin J Huebner Kay K Linster Carole L CL Cooper Arthur J L AJL Van Schaftingen Emile E
Proceedings of the National Academy of Sciences of the United States of America 20170403 16
The mammalian gene <i>Nit1</i> (nitrilase-like protein 1) encodes a protein that is highly conserved in eukaryotes and is thought to act as a tumor suppressor. Despite being ∼35% sequence identical to ω-amidase (Nit2), the Nit1 protein does not hydrolyze efficiently α-ketoglutaramate (a known physiological substrate of Nit2), and its actual enzymatic function has so far remained a puzzle. In the present study, we demonstrate that both the mammalian Nit1 and its yeast ortholog are amidases highly ...[more]