Ontology highlight
ABSTRACT:
SUBMITTER: Ece S
PROVIDER: S-EPMC5403781 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
Ece Selin S Lambertz Camilla C Fischer Rainer R Commandeur Ulrich U
AMB Express 20170424 1
Laccases are used for the conversion of biomass into fermentable sugars but it is difficult to produce high yields of active laccases in heterologous expression systems. We overcame this challenge by expressing Streptomyces cyaneus CECT 3335 laccase in Escherichia coli (ScLac) and we achieved a yield of up to 104 mg L<sup>-1</sup> following purification by one-step affinity chromatography. Stability and activity assays using simple lignin model substrates showed that the purified enzyme preparat ...[more]