Dataset Information
Heterogeneity of hypothalamic pro-opiomelanocortin-expressing neurons revealed by single-cell RNA sequencing.
Ontology highlight
ABSTRACT: Objective
Arcuate proopiomelanocortin (POMC) neurons are critical nodes in the control of body weight. Often characterized simply as direct targets for leptin, recent data suggest a more complex architecture.Methods
Using single cell RNA sequencing, we have generated an atlas of gene expression in murine POMC neurons.Results
Of 163 neurons, 118 expressed high levels of Pomc with little/no Agrp expression and were considered "canonical" POMC neurons (P+). The other 45/163 expressed low levels of Pomc and high levels of Agrp (A+P+). Unbiased clustering analysis of P+ neurons revealed four different classes, each with distinct cell surface receptor gene expression profiles. Further, only 12% (14/118) of P+ neurons expressed the leptin receptor (Lepr) compared with 58% (26/45) of A+P+ neurons. In contrast, the insulin receptor (Insr) was expressed at similar frequency on P+ and A+P+ neurons (64% and 55%, respectively).Conclusion
These data reveal arcuate POMC neurons to be a highly heterogeneous population. Accession Numbers: GSE92707.
SUBMITTER: Lam BYH
PROVIDER: S-EPMC5404100 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Publications
Heterogeneity of hypothalamic pro-opiomelanocortin-expressing neurons revealed by single-cell RNA sequencing.
Molecular metabolism 20170301 5
<h4>Objective</h4>Arcuate proopiomelanocortin (POMC) neurons are critical nodes in the control of body weight. Often characterized simply as direct targets for leptin, recent data suggest a more complex architecture.<h4>Methods</h4>Using single cell RNA sequencing, we have generated an atlas of gene expression in murine POMC neurons.<h4>Results</h4>Of 163 neurons, 118 expressed high levels of <i>Pomc</i> with little/no <i>Agrp</i> expression and were considered "canonical" POMC neurons (P<sup>+< ...[more]
