Specific and quantitative detection of Human polyomaviruses BKPyV and JCPyV in the healthy Pakistani population.

ABSTRACT: The BK Polyomavirus (BKPyV) and JC polyomavirus (JCPyV) infections are widespread in human population and have been associated with severe kidney and brain disorders, respectively. The viruses remain latent primarily in reno-urinary tract, reactivating only in case of a compromised immune system. The seroepidemiology and molecular prevalence of BKPyV and JCPyV have been widely studied both in healthy and immunocompromised patients worldwide. However, data regarding the prevalence of these viruses in the immunocompetent or apparently healthy Pakistani population is lacking. Herein, we present the first ever report on quantitative prevalence of BKPyV and JCPyV in the peripheral blood of a randomly selected cohort of healthy Pakistani population.A total of 266 whole blood samples were examined. The subjects were divided into three age groups: ? 25 years (young), 26-50 years (middle) and???51 years (elder). Absolute real time PCR assay was designed to quantify the BKPyV and JCPyV viral copy numbers in the range of 106 to 100 copies/mL.Overall, BKPyV was detected in 27.1% (72/266) individuals while JCPyV in 11.6% (31/266) indicating significant difference (p?

SUBMITTER: Hussain I 

PROVIDER: S-EPMC5404684 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature