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Stella modulates transcriptional and endogenous retrovirus programs during maternal-to-zygotic transition.


ABSTRACT: The maternal-to-zygotic transition (MZT) marks the period when the embryonic genome is activated and acquires control of development. Maternally inherited factors play a key role in this critical developmental process, which occurs at the 2-cell stage in mice. We investigated the function of the maternally inherited factor Stella (encoded by Dppa3) using single-cell/embryo approaches. We show that loss of maternal Stella results in widespread transcriptional mis-regulation and a partial failure of MZT. Strikingly, activation of endogenous retroviruses (ERVs) is significantly impaired in Stella maternal/zygotic knockout embryos, which in turn leads to a failure to upregulate chimeric transcripts. Amongst ERVs, MuERV-L activation is particularly affected by the absence of Stella, and direct in vivo knockdown of MuERV-L impacts the developmental potential of the embryo. We propose that Stella is involved in ensuring activation of ERVs, which themselves play a potentially key role during early development, either directly or through influencing embryonic gene expression.

SUBMITTER: Huang Y 

PROVIDER: S-EPMC5404928 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

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Stella modulates transcriptional and endogenous retrovirus programs during maternal-to-zygotic transition.

Huang Yun Y   Kim Jong Kyoung JK   Do Dang Vinh DV   Lee Caroline C   Penfold Christopher A CA   Zylicz Jan J JJ   Marioni John C JC   Hackett Jamie A JA   Surani M Azim MA  

eLife 20170321


The maternal-to-zygotic transition (MZT) marks the period when the embryonic genome is activated and acquires control of development. Maternally inherited factors play a key role in this critical developmental process, which occurs at the 2-cell stage in mice. We investigated the function of the maternally inherited factor Stella (encoded by <i>Dppa3</i>) using single-cell/embryo approaches. We show that loss of maternal Stella results in widespread transcriptional mis-regulation and a partial f  ...[more]

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