Unknown

Dataset Information

0

Global reorganisation of cis-regulatory units upon lineage commitment of human embryonic stem cells.


ABSTRACT: Long-range cis-regulatory elements such as enhancers coordinate cell-specific transcriptional programmes by engaging in DNA looping interactions with target promoters. Deciphering the interplay between the promoter connectivity and activity of cis-regulatory elements during lineage commitment is crucial for understanding developmental transcriptional control. Here, we use Promoter Capture Hi-C to generate a high-resolution atlas of chromosomal interactions involving ~22,000 gene promoters in human pluripotent and lineage-committed cells, identifying putative target genes for known and predicted enhancer elements. We reveal extensive dynamics of cis-regulatory contacts upon lineage commitment, including the acquisition and loss of promoter interactions. This spatial rewiring occurs preferentially with predicted changes in the activity of cis-regulatory elements and is associated with changes in target gene expression. Our results provide a global and integrated view of promoter interactome dynamics during lineage commitment of human pluripotent cells.

SUBMITTER: Freire-Pritchett P 

PROVIDER: S-EPMC5407860 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications


Long-range <i>cis-</i>regulatory elements such as enhancers coordinate cell-specific transcriptional programmes by engaging in DNA looping interactions with target promoters. Deciphering the interplay between the promoter connectivity and activity of <i>cis-</i>regulatory elements during lineage commitment is crucial for understanding developmental transcriptional control. Here, we use Promoter Capture Hi-C to generate a high-resolution atlas of chromosomal interactions involving ~22,000 gene pr  ...[more]

Similar Datasets

2017-03-23 | GSE86821 | GEO
| S-EPMC1829156 | biostudies-literature
| S-EPMC4123067 | biostudies-literature
| S-EPMC4115678 | biostudies-literature
| S-EPMC3191068 | biostudies-literature
| S-EPMC3624936 | biostudies-literature
| S-EPMC2442567 | biostudies-other
| S-EPMC2329687 | biostudies-literature
| S-EPMC7684319 | biostudies-literature
| S-EPMC4060806 | biostudies-literature